solanum tuberosum lectin  (Vector Laboratories)

Journal: GeroScience

Article Title: Effect of high-fat diet on cerebral pathological changes of cerebral small vessel disease in SHR/SP rats

doi: 10.1007/s11357-024-01074-7

Figure Lengend Snippet: HFD-induced small vessel density and diameter in the cerebral of WKY, SHR, and SHR/SP rats. A Cerebral vessels were revealed after Solanum Tuberosum lectin (STL) immunostaining in the frontal cortex, corpus callosum, and striatum regions of WKY, SHR, and SHR/SP rats fed ND or HFD. Scale bar = 50 μm. B Statistical analysis results of microvessel numbers in the frontal cortex, corpus callosum, and striatum. The microvessel density in the frontal cortex region significantly increased in HFD-WKY, HFD-SHR, and HFD-SHR/SP rats compared to their respective ND groups. C Statistical analysis results of capillary diameter in the frontal cortex, corpus callosum, and striatum regions. Capillary diameters in the frontal cortex were notably wider in HFD-fed SHR/SP rats compared to their ND-fed counterparts, and wider in both HFD-fed SHR and HFD-fed SHR/SP rats compared to HFD-fed WKY rats. In the corpus callosum region, capillary diameter was broader in HFD-fed SHR rats compared to ND-fed SHR rats. In the striatum region, capillary diameters were wider in ND-fed SHR/SP rats compared to ND-fed SHR rats. Results are means ± SD (n = 3, 3 FOV for each region). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the corresponding results of the ND group. In the ND-fed group, ###P < 0.001 vs. the ND-WKY rats. In the ND-fed group, ††P < 0.01 vs. the ND-SHR rats. In the HFD-fed group, △P < 0.05, △△P < 0.01, △△△P < 0.001 vs. the HFD-WKY rats. FOV: field of view

Article Snippet: Immunofluorescent (IF) staining The procedure and protocol for IF staining have been reported in our previous studies [ 27 , 28 ] and this study was performed on 10 µm frozen sections. . Antibodies Antibodies reactive for the following antigens were used in this study: Solanum tuberosum lectin (STL, 1:200, Vector Laboratories, Newark, CA, USA), anti-Collagen IV IgG (rabbit polyclonal, 1:200, Novus Bio Co. Ltd., Colorado Spring, CO, USA), anti-Claudin 5 IgG (rabbit polyclonal, 1:200, Abcam Co. Ltd., Cambridge, UK), anti-Iba-1 IgG (rabbit polyclonal, 1:200, Abcam Co. Ltd., Cambridge, UK), anti-GFAP IgG (rabbit polyclonal, no dilution, Dako, Agilent Co. Ltd., Carpinteria, CA, USA), anti-GP91-phox IgG (mouse monoclonal, 1:200, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-HIF-1α IgG (mouse monoclonal, 1:50, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Immunostaining

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Clinical characteristics of patients for lectin microarray.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques: Microarray

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Clinical characteristics of patients for lectin blot.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques:

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Lectin array profiling of serum samples from Hashimoto′s thyroiditis (HT) patients (n=27) and healthy control (HC) (n=26). (A) Representative results of the array profiling for HT (left) and HC (right) groups. Red boxes indicate the position of lectins with the significant difference between groups. (B) Principal component analysis (PCA) of the mean normalized signals obtained from lectin microarray. A scatter plot for principal component (PC) 1 and 2 is shown. Each dot represents the sample derived from an individual person. Different groups are indicated by color. (C) Volcano plot of log2 fold change (x axis) in lectin binding signals of HT samples vs . HC samples against the significance of change shown as the −log10 p-value (y axis). Red and green dots indicate lectins with up-regulated and down-regulated binding signals, respectively. (D) Heat map and hierarchical clustering of lectin microarray signals. Each column represents samples from an individual person. Thirteen lectins are shown on rows. (E) The binding signal of 14 lectins shows a significant difference between groups. *p <0.05, **p <0.01, ***p <0.005 and ****p <0.001. VVA, Vicia villosa lectin; MAA, Maackia amurensis lectin; STL, Solanum tuberosum lectin; Calsepa, Calystegia sepium lectin; SNA-I, Sambucus nigra I lectin; SAMB, Sambucus Sieboldiana lectin; DSA, Datura stramonium lectin; BanLec, Musa acuminata lectin; ACG, Agrocybe cylindracea lectin; PHA-E, Phaseolus vulgaris Erythroagglutinin ; MPL, Maclura pomifera lectin; PSA, Pisum sativum lectin; Gal1, Human galectin1 lectin; GS-I, Griffonia simplicifolia I lectin.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques: Control, Microarray, Derivative Assay, Binding Assay

Journal: Frontiers in Immunology

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins

doi: 10.3389/fimmu.2023.1182842

Figure Lengend Snippet: Lectin blot verification of a small cohort of serum samples profiled by lectin array. (A) The VVA, MAA, STL, SNA-I and DSA blot using serum samples obtained from the HT (left 2-8 column) and HC group (left 9-15 column). The number above each column indicates the corresponding sample. VVA and MAA bands are shown at ~75 KDa and 100 KDa, respectively. Band for STL blot was between 75 to 100 KDa on SDS-PAGE. SNA-I and DSA bands are shown at ~60 KDa. (B) The signal intensity analysis of bands from VVA, MAA, STL, SNA-I and DSA blot. All error bars indicate SD. *p <0.05.

Article Snippet: The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark.

Techniques: SDS Page

Journal: bioRxiv

Article Title: Exploring the vomeronasal organ in an endangered antelope species

doi: 10.1101/2023.03.09.531847

Figure Lengend Snippet: All the lectins employed labelled the vomeronasal glands (VG) and the muco-microvillar complex, i.e. the superficial border of both vomeronasal epithelia. A, E . Labelling of the VNO at levels L3 and L8, respectively with the lectin SBA ( Glycine max ). In both cases the VG are strongly labelled. B. VVA ( Vicia villosa ) labelling at a central level L6 of the VNO shows a faint labelling of the VG. C, D. LEA ( Lycopersicon esculentum ) labels the vomeronasal epithelia, the NVNs, and the VG. LEA does not stain the caudal nasal nerve (asterisk in C). F, I, K, N. STA ( Solanum tuberosum ) lectin labelling at central levels (L4, L5) of the VNO stains the vomeronasal epithelia, vomeronasal nerves (NVN) and VG. G, H, J, L, M. UEA ( Ulex europaeus ) lectin stains vomeronasal epithelia, VG, but hardly stains NVNs. Vv, veins; directionality is indicated by the following: d, dorsal; l, lateral; m, medial; v, ventral. Scale bars: A-C, E, I, J = 500 μm; D, F, G = 250 μm; H = 100 μm; K-N = 50 μm.

Article Snippet: STA (Vector-B1165), which is isolated from potato, Solanum tuberosum , binds oligomers of N-acetylglucosamine and some oligosaccharides containing N- acetylglucosamine and N-acetylmuramic acid ( ).

Techniques: Staining